nebnext ultra ii end repair da  (New England Biolabs)

Journal: bioRxiv

Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function

doi: 10.64898/2026.02.16.706237

Figure Lengend Snippet: (A) Expression of total Xbp1 mRNA (left) and spliced Xbp1 ( Xbp1s ) mRNA (right) in NFs and CAFs transduced with either empty vector (EV) or sh Xbp1 constructs, n=3. (B) IF staining of collagen I (green) in NFs and CAFs transduced with EV or sh Xbp1 constructs, and quantification of collagen I high-density matrix area normalised to cell number. Nuclei are stained with DAPI (blue). Scale bars represent 10 µm.

Article Snippet: Cell suspension was combined with 400 μL 3 mg/mL rat tail collagen I solution (Thermo Fisher Scientific) and immediately neutralized with 12 μL of sterile 10 mM NaOH solution.

Techniques: Expressing, Transduction, Plasmid Preparation, Construct, Staining

Journal: bioRxiv

Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function

doi: 10.64898/2026.02.16.706237

Figure Lengend Snippet: Representative IF staining of collagen I (green) in (A) NFs and CAFs treated with DMSO (Ctrl) or 1 µM Sim for 48 hours, or (B) NFs and CAFs transduced with empty vector (EV) or sh Hmgcr constructs, and quantification of collagen I high-density matrix area normalized to cell number. Nuclei are stained with DAPI (blue). Scale bars represent 10 µm. ECM remodelling capacity of (C) NFs and CAFs treated with DMSO (Ctrl) or 1 µM Sim for 48 hours, or (D) NFs and CAFs transduced with EV or sh Hmgcr constructs as determined by a collagen contraction assay over 24 hours. Data are represented as % contraction relative to NF Ctrl (C) or NF EV (D) , n=3.

Article Snippet: Cell suspension was combined with 400 μL 3 mg/mL rat tail collagen I solution (Thermo Fisher Scientific) and immediately neutralized with 12 μL of sterile 10 mM NaOH solution.

Techniques: Staining, Transduction, Plasmid Preparation, Construct, Contraction Assay